E. Coli DNA Topoisomerase I
14K C-terminal fragment
The C-terminal fragment of topoisomerase I interacts with both single- and double-stranded DNA and enhances DNA-binding activity of the holoenzyme. This fragment also enhances the enzyme's ability to relax negative supercoils in DNA, presumably by strengthening DNA-binding.
Several topoisomerase residues implicated in binding single-stranded DNA <