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Instructor: Pat Heithaus Ryn Edwards Ray Heithaus Karen Hicks Harry Itagaki
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1. What is the function of restriction enzymes?
2. How do DNA molecules with "sticky ends" differ from other DNA molecules?
3. What are two characteristics of pK19 that make it a good vector for bacterial transformation?
4. If bacteria are transformed by a foreign plasmid how will you know this has happened?
5. How do bacteria that are transformed by plasmids with Rhodobacter sphaeroides DNA differ from bacteria that are transformed by a plasmid lacking foreign DNA.
they can grow on kanamycin they can produce functional beta galactosidase they do not have an intact alpha peptide two of the above