Cloning Quiz




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Fill in the answers to the questions below and submit a copy of this form before attending lab.

1. What is the function of restriction enzymes?

2. How do DNA molecules with "sticky ends" differ from other DNA molecules?

3. What are two characteristics of pK19 that make it a good vector for bacterial transformation?

4. If bacteria are transformed by a foreign plasmid how will you know this has happened?

5. How do bacteria that are transformed by plasmids with Rhodobacter sphaeroides DNA differ from bacteria that are transformed by a plasmid lacking foreign DNA.