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Instructor: Pat Heithaus Ryn Edwards Chris Gillen Jen Smith Wade Powell
IMPORTANT PRE LAB INSTRUCTIONS: PCR can be inhibited by the caffeine in coffee or tea, as well as tars in cigarette smoke. Please refrain from consuming caffeinated bevrages or smoking at least several hours before coming to lab. This will increase the probablility your DNA can be amplified.
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1. Why is the polarity of the two strands of DNA important and how is this related to the manner in which replication occurs?
2. What are the complementary base pairs in the DNA molecule?
3. Make up a 4 nucleotide sequence and show what a 3 VNTR of your sequence would look like.
4. What role do each of the following play in PCR - dNTPs, taq polymerase, primers?
5. Which of the following statements about primers used in PCR is not true?
they bracket the region of interest they both complement the DNA of the same strand of the template they attach at the 3' end of the template strand their base pair sequence is known