Name:
Email:
Instructor: Pat Heithaus Ryn Edwards Jennifer Smith Wade Powell
Use the back/forward buttons on your computer if you want to move between the tutorial and this document
1. Briefly explain the purpose of running an SDS-PAGE gel.
2. What direction do the proteins in the wells of and SDS-PAGE gel move? a. horizontally across the gel b. toward the positive end of the gel c. vertically down the gel d. two of the above
3. Another of the steps in a Western Blot also uses electrophoresis. What is the major purpose of this step?
Below are several true and false questions. Indicate if they are true or false.
4. Primary antibodies are specific for the protein being investigated
5. Primary antibodies are produced when rabbit proteins are injected into a rabbit
6. Blocking with milk increases the possibility of non-specific protein binding by the primary antibody
7. Secondary antibodies respond to any antibody produced by the organism that made the primary antibody.
8. Secondary antibodies cause all the proteins in the blot to turn blue
9. Alkaline phosphatase causes proteins to turn red.
10. Alkaline phosphatase does not prevent the binding of the secondary antibody.