Introduction to Experimental Molecular Biology
Biology 111 introduces students to important concepts and techniqes used in modern molecular biology labs. The course follows Biology 109 in the introductory laboratory sequence as an alternative to Biology 110. After technical training through planned laboratory exercises during the first two-thirds of the course, students will design, conduct, and present the results of an independent research project.
Students will gain extensive experience in many important molecular biology techniques, including (but not limited to):
- Isolation, quantitation, and manipulation of distinct DNA molecules.
- Amplification of specific DNA sequences using the polymerase chain reaction (PCR).
- Electrophoresis of nucleic acids.
- Overexpression of proteins in bacterial hosts.
- Electrophoresis of proteins.
- Detection and quantitation of protein expression using western blots.
- Bioinformatics: The use of computers and public databases to obtain and analyze DNA and protein sequence information.
- The arts-and-crafts of presenting scientific data.
- Scientific writing
Molecular Biology Major