BIOL 114 Home

Early animal development I: fertilization

A scanning electron micrograph of human sperm and oocyte


Fertilization: Acrosomal reaction


from the Sea Urchin Embryology Tutorial
 



Fast block to polyspermy


from LIFE: The Science of Biology, Purves et al, 1998



Slow block to polyspermy


from LIFE: The Science of Biology, Purves et al, 1998

Click on the image below to watch a movie showing the events involved in the slow block to polyspermy! This movie shows simultaneous double imaging of phase contrast (left) and intracellular Ca ++ concentration (right) during sea urchin fertilization. The left image shows the approach of the sperm at about 2 o'clock and the rising of the vitelline membrane. Intracellular Ca++ is monitored by an indicator that becomes more fluorescent when it binds Ca++. A transient Ca++ rise around the entire cortex (fifth frame) is followed by the Ca++  wave, which begins at the sperm entry site.

image and movie from Mark Terasaki