BIOL 114
Biology Dept
Kenyon College
Homework 3

1.  Solve two Plasmid maps, one Easy, one Intermediate.  Print out the solution pages to submit with your work.

2.  Here is the annotated DNA sequence of the virulence plasmid for Bacillus anthracis.
How many proteins does it encode?  (Display, Graphics)

Perform a restriction analysis on the first three thousand bases of DNA of this plasmid.
To do this, paste the DNA sequence (at the bottom of the Display, Default page) into Webcutter (where it says, Paste the DNA sequence into the box below.)
Which enzymes cut only once?  [Hint: Pick this option in Webcutter.]
Why would they be useful in cloning?
Where does EcoRI cut the sequence?