|
||
Dr. Joan
Slonczewski
with
Darcy Blankenhorn
|
Under continual revision; Check for updates |
Spring
Semester
2014, HIG 322 |
Microbiology is the study of organisms too small to
be seen by the naked eye. In this experimental lab course,
we observe bacteria, protists, and archaea. Our experimental
approaches include:
Prerequisite courses. BIOL 109
is required. BIOL 238 must be taken concurrently or
previously.
Lab
hours. Lab starts in HIG 322 Thursday at 1:10. Lab Procedures are linked on line for each lab. Always check the blackboard for modifications. Text: Slonczewski &
Foster (2014) Microbiology: An Evolving Science
(Norton, 3E). Lab
reports. Reports are due on Moodle by 11:59
midnight of the date shown.
Evaluation.
Your final grade is based on lab reports: Attendance.
All missed labs will count against your grade. Lab
attendance is 100% absolutely required. Special needs. The Coordinator of Disability Services must be consulted for special arrangements. No Withdrawals. Passing this course assumes 100%
participation up to and including the final lab. |
Date
|
Topic
|
Prep
Reading
|
Due by
11:59pm |
|
Jan
|
16
17
|
Lab
1. Visualizing eukaryotes. Pour plates.
Complete
observations as needed.
|
Lab
1; S&F Ch 2, Ch 20 |
1/21
|
Jan
|
23
24
|
Complete
as needed.
|
Lab 2; S&F Ch 4 HMP 2012 |
1/28
|
Jan
|
30
31
|
Lab 3; S&F Ch 5 TolC |
2/12
|
|
Feb
|
06
07 |
Lab
3 (cont). Report due 2/12 by 11:59pm. |
||
Feb
|
13
14 |
No class. |
|
|
Feb
|
20
21 |
Lab 4 |
Lab 4; S&F Ch 8 Desai Arabinose |
2/27
|
Febr
|
24
27 |
Monday:
Check restreaked plates [if needed].
Lab 4 DUE -- Hospital Field Trip. |
S&F Ch 28 |
|
|
||||
Mar
|
20
21
|
Lab 5; Jeon; Shade SF Ch 18, 21 |
4/16;
5/1 |
|
Mar |
26 27 28 |
Wednesday: Inoculate 15
tubes from isolated colonies. DNA preparation. Complete DNA prep. |
DNA prep | |
Apr | 02 03 04 |
PCR amplification of DNA for sequencing. Thursday AM: DNA gel. Thursday PM: Purify PCR. (Ship bands to OSU DNA Sequencing) |
PCR prep | |
Apr
|
10
|
DNA
BLAST. Plan
Physiology tests |
||
Apr
|
17
|
Antibiotic
producers & resistance; Physiology tests; |
Lab 5 Antibiotic | 4/16 5A DUE |
Apr
|
24
|
Followup tests. Hugenholtz. |
- |
|
May
|
01
|
Lab
5 Presentations. Water
Treatment Plant Field Trip
|
S&F pp 839-841 |
Lab 5 Full
|
The
purpose of these rules is to keep you and your fellow
students safe and healthy. Remember that in a microbiology
lab, any microorganism is considered a potential pathogen.
Observance of safety rules will be a factor in your lab
grade. Remember to assume that any microbe is a potential pathogen. Eating, drinking and chewing gum are not allowed in the lab. Read the day’s procedure for the lab exercise and the appropriate report pages before you come to class. Before the lab period begins, check the lab blackboard for any modification of your procedure. Wait for the instructor to arrive before beginning work. Protect your microscope. Always carry with both hands; grasp the arm with one hand, and support the base with the other hand. Keep your microscope covered when not in use. Never remove or exchange eyepieces with other microscopes. Avoid excess immersion oil. Always clean oil off lenses and slides when you are finished. Use only lens paper with 70% ethanol to clean lenses; blot, not rub. Do not pour liquefied agar medium down the sink drain, as it will cool and gel in the pipes. Do not pour any contaminated media down the drain; instead, leave it in the prep room for autoclaving and safe disposal. Your lab area must be left clear and disinfected with ethanol before you leave the lab. Hang up your lab coat before you leave the lab. |