Find a Human gene in OMIM, NCBI, and ENSEMBL. Align with the mouse ortholog.

1. OMIM: Search a particular disease, such as Cystic Fibrosis.
Click on one gene associated with the disease. Tour information. How does it relate to disease? Are mutant forms known?

2. Click Gene map locus, such as 7q31.2. Gene appears in a table, with other nearby loci arranged in order on the chromosome.

3. Click Location (column at left), i.e. 7q31.2. This takes you to NCBI Map Viewer. Your selected gene is highlighted pink.

4. Click your gene on each information column where it is available (Find it first): Gene_seq; you will have to click to make a new gene link appear.
C lick the sv link to sequence viewer. Here, you can scroll back and forth along the chromosome. What kinds of information appear?
Afterward, use Back button to return to Map Viewer.

5. In the pink highlighted region, right of the OMIM link, try pr, hm, sts. What kind of information do you find?

6. In Map Viewer, in the blue-gray column at left, click Maps & Options (yellow letters). Use option box (left) to add Ensemble Genes. Click OK (lower left). Now a new map will appear (next to Morbid). Find your gene on this map (should be very small, pink highlight). Click; new link appears; click again. Now you have switched to Ensembl database, for example CFTR. Alternative, the main Ensembl page is here.

7. In Ensemble, find transcript(s) of your gene. Where are the exons and introns?
View Alignment with another animal genome, or with several at once. How similar are the orthologs?

Compare genes of two bacterial species in CMR.
CMR: Compares microbial genomes.
Scroll and click: Lists; Gene Lists;Genes by Role Category.
Select a category of interest such as Cellular Processes; Pathogenesis.
Select two genomes (such as E. coli O157 and Bacillus anthracis). "Add" each to right.
Note: Plenty of other pathogens may be selected instead.
Click Submit.
Scroll information. What kinds of pathogenesis genes do the organisms share?
Pick a non-pathogen for comparison, such as Synechococcus or Sulfolobus.. Are the genes still shared? Why or why not?