BIOL 264: Gene Manipulation
Dr. Wade H. Powell
FSH 202; PBX5396
Fall Semester 2009
Current Syllabus (KC Moodle Site; campus access only--Sorry!)
Archived Fall 2006 syllabus (Publicly available)
This course introduces students to molecular biology research through a collaborative, semester-long group project. The planned project is designed to enable students to work independently, competently, and safely in a molecular biology laboratory.
The goal of the project is to clone cDNAs encoding Cytochrome P4501A (CYP1A) enzymes from several vertebrate species. We will use the sequences of the cDNA clones we obtain to: (1) construct the phylogenetic history of the protein during vertebrate evolution, and (2) to study of effect of environmental contaminants on the expression of the associated mRNAs.
Students will gain extensive experience in experimental design, performance, and data analysis from many important molecular biology techniques, including :
* Isolation of nucleic acids from animal tissues.
Students will develop the ability to produce high-quality scientific writing that demonstrates:
* Lucid prose, including strong sentence construction, well developed paragraphs, and logical organization.