pKC105

This recombinant plasmid pKC105 contains both plasmid DNA and DNA from R. sphaeroides. Compare it to the original pK19 plasmid. The plasmids you are working with today were derived from this plasmid by using addtional restriction enzymes to cut the plasmid and isolate novel regions of DNA. Step 3. Once you have created the plasmid it is necessary to determine if you have plasmids with the insert, reannealed plasmids (like the original), or other unexpected plamids. Step 4. Transformation of bacteria using the mixture of plasmids.