Amount of 2.5 mM ONP to combine with pH 7.7 Buffer to Achieve the Desired ONP Concentration

Desired final concentration

Amount of 2.5 mM ONP stock solution (ml)

pH 7.7 Buffer
(ml)

.175 mM

0.7

9.3

.125 mM

0.5

9.5

.075 mM

0.3

9.7

.025 mM

0.1

9.9

Make sure you are comfortable with the above calculations