Acetaldehyde/PKC Questions

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Instructor: Chris Gillen Ryn Edwards Pat Heithaus Wade Powell Jennifer Smith

You will need to use Moodle to access a copy of :

Wyatt, T. A., S. C. Schmidt, S.I. Rennard, D.J. Tuma, and J. H. Sission. 2000. Acetaldehyde-stimulated PKC activity in airway epithelial cells treated with smoke extract from normal and smokeless cigarettes. P.S.E.B.M. 225: 91-97.

Figure1. Hypotheses to explain relationship between cigarette smoke and cilliary beat frequency (CBF) as suggested by Wyatt et al. (2000).

Fill in the answers to the questions below and submit a copy of this form to your instructor before attending lab.

Introduction - this section develops background that leads to the hypotheses that are going to be tested.

What are the four major points made in the first paragraph? Begin with the topic sentence. In answering this question, be sure that you establish the suspected link between CBF, acetaldehyde, and PKC and define each term. (Paragraph 1)

1.

2.

3.

4.

5. What are three known facts about acetaldehyde? (Paragraph 2)

6. Why include smoke extracts from both regular and Eclipse cigarettes as part of the experimental design? (Paragraph 3)

7. What hypotheses are the authors testing?

Results

8. How did the concentration of acetaldehyde affect the CBF of bovine bronchial epithelial cells (Figure 1)?

9. Which of the three treatments caused the greatest increase in PKC activity during the first hour of the experiment (Figure 2)?

media CSE (5%) ESE (5%)

10. Which of the three treatments showed a time-dependent activation of PKC activity (Figure 3)?

5% ESE 10%ESE 5%CSE

11. Acetaldehyde can be lost from extracts or treatment media. Under what condition is the level of acetaldehyde apt to be the highest (figure 5)?

ESE sealed ESE CSEsealed CSE

12. HBBDE inhibits PKC activity. Compare the CBF response of control cultures and those treated with CSE or CSE + HBBDE. What do the differences suggest (Figure 7)?