Toxins and Ciliary Movement Questions

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Instructor: Chris Gillen Ryn Edwards Pat Heithaus Wade Powell Jennifer Smith

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Before answering these questions you should have completed the tutorial and read the following paper which is available on the Biology 109 Moodle site:

Stefanidou M., M. Georgious, C. Maravelias and A. Koustelinis. 1990. The effects of morphine, cocaine, amphetamine and hashish on the phagocytosis of the protozoon Tetrahymena pyriformis strain W. Toxic. in Vitro 4 (6): 779-781.

Fill in the answers to the questions below and submit a copy of this form toyour instructor before attending lab.

1. What do the cells lining lungs and Tetrahymena have in common?

2. What forms the 9+2 pattern of cilia?

spoke proteins contractile proteins cytoplasm bundles of microtubules

3. Which protein is primarily responsible for the bending of the cilia?

nexin spoke proteins dynein bridge proteins

4. What factors make Tetrahymena a good model organism to use in exploring the effects of cigarette smoke?

5. Why might serial dilution be used when designing an experiment on the effects of cigarette extracts?

6. Adding 1ml of extract to 99ml of water results in what level of dilution?

7-10. Indicate the four major parts of a scientific paper and the general type of information that is included in each section.

11. References to the literature are included in both the introduction and discussion sections of a scientific paper. The role they play in the two sections differs slightly. Explain this difference.

12. What are the two major bits of information given when citing literature in the text of a scientific paper?

Statistics question of the week

13. You are interested in determining how time affects the rate at which food vacuoles form in Tetrahymena, so you design an experiment that has three groups. One group is examined immediately after ink is added as a food source, the second is examined 15 minutes after ink is added, and the third is examined 30 minutes after ink is added. What statistical test would you use to determined if time (a single variable) affects the number of food vacuoles? Why? If you need help go to the statistics flow chart then return to this page using the Back Button.