|
Biol 111
Subject
to change; |
| PART I |
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| Jan 22 | Course Introduction Lab Procedures and Lab Safety Care and use of the Rainin Pipetman Problem Set: Common Calculations in the Molecular Biology Laboratory Ligation of DNA insert into Plasmid Vector | |
| Jan 29 | Use Ligation Reactions to Transform
Competent E. coli cells Go over Problem Set |
|
| Feb 5 | Plasmid minipreps Assessment of Recombinant Plasmids by Restriction Enzyme Digestion Restreaking Miniprep Cultures |
Preliminary Project Proposals Due |
| Feb 12 | Agarose Gel Electrophoresis of
Digested Plasmids Digital Photography and Preparation of Figures |
|
| PART II |
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| Feb (18 and) 19 | Bacterial Cell Culture
Induce Expression of Recombinant Protein (GST and GST Fusion) Preparation of Samples for Electrophoresis |
First Lab Report Due: Construction of Recombinant Plasmids |
| Feb 26 | SDS Gel Electrophoresis
Western Blotting |
|
| March (3 and) 4 | Probing Blots with Anti-GST Antibody
Development of Western Blot |
Final Project Proposals Due |
| March 11
March 18 |
SPRING BREAK | |
| PART III |
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| March 25 | Human DNA Isolation PCR Amplification of Microsatellite DNA Review Western Blot Results |
|
| April 1 | Agarose Gel Electrophoresis of
PCR Products Data Analysis |
Second Lab Report Due: Detection of Recombinant Protein Expression By Western Blotting |
| PART IV |
|
Guidelines and Deadlines |
| April 8 | Begin Exclusive Work on Independent Projects | |
| April 15 | Independent Projects Continue | Third Lab Report Due: DNA Fingerprinting |
| April 22 | Independent Projects Continue | |
| April 29 | Independent Projects Continue | |
| May 6 | Oral Presentations on Independent Projects | |
| **May 11 (Tuesday)** | *****Turn In Final Project Reports: 9:30 PM***** |