BIOL 114 Biology Dept Kenyon College

1.  To begin DNA replication, what enzyme first binds to DNA?
  DNA polymerase
  Helicase

2.  What happens to the DNA molecule?
  It unwinds.
  It winds more tightly.

3.  Can DNA replication start anywhere?
  Yes.
  Only at an origin site (ori).

4.  What happens to the single-stranded DNA?
  It gets cut by enzymes.
  It gets protected by single-strand DNA binding proteins.

5.  The helicase binds at:
  A replicating fork (two are generated by each ori site).
  All the single-stranded DNA.