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EXAM III: Points to ponder

Topics Emphasized since Exam II:

Structure and Function of DNA polymerase III;
Trombone model of replication, structure and function of subunits of the holoenzyme.

Sources and types of DNA damage; repair schemes, including mechanisms and components, for each. 
Special emphasis on MutS structure and function (see Obmolova 2000 paper).

Homologous recombination:

• Holliday model of strand invasion and resolution of junctions for single and double strand breaks.
• Structure and function of proteins that mediate the recombination process in prokaryotes and eukaryotes.

Telomeres and telomerase:

• Telomeres:  structure and purpose.
• Telomerase:  structure and function.
• How telomere length and capping state relate to cell division (Blackburn 2000 review).
• Cancer cell growth and telomerase knockdown (Li et al. 2004 paper)

DNA sequencing chemistry: Sanger and "next generation"
Bioinformatics and genome annotation.
Mapping vs. sequencing.

Expression microarray technologies:  

• How they work and how they’re useful. 
• Data handling and analysis
• Research: Schaffer et al. 2001 microarray paper.

Transgenic and knockout mouse methods (?).