Fall Semester 2005
Activities subject to the uncertainties of science.
Deadlines are firm.
Updated Oct. 13, 2005
NOTE: Readings and handouts are available by direct link or via e-res, the electronic reserve system of the CONSORT libraries.
|Sept. 1||Course introduction; pipettor verification; quantification of nucleic acids by spectrophotometry; intro. to degenerate primer design||Morrison et al. (1995)
Morrison et al. (1998)
Iwata and Stegeman (2000)
Wilkie and Simon (1991)
|Sept. 8||Total RNA Isolation, quantitation.||RNA isolation protocol|
|Sept. 15||RNA gels, photography; Figure preparation;
Workshop: Primer design.
|Wilkie and Simon (1991)||Homework: primer sequences|
(Meet in Computer Lab, FSH 009)
|Computer Workshop: Bioinformatics BLAST searches, sequence alignment, phylogenetic trees.||
Handout: DNA and Protein Sequence analysis.
|Lab Report 1: RNA Isolation
Also: Notebook check.
|Sept. 29||RT-PCR for CYP1A cDNA fragments||RT-PCR Protocols|
|Oct. 6||Agarose gel analysis of PCR products|
|Oct. 13||Purification of RT-PCR products from gels; ligation of products into plasmid cloning vector.||Gene Clean protocol
|Lab Report 2: Sequence analysis exercise.|
|Oct. 20 (and 21)||Transformation of competent E. coli cells.|
|Oct. (26 and) 27||Plasmid minipreps; restriction enzyme digestion.||Miniprep protocol
Restriction ezyme protocol
|Notebook Check by request.|
|Nov. 3||Gel analysis of miniprep digests.
Writing workshop: Guided peer review previous lab report.
Workshop: Intro to Real-Time PCR;
|Applied Biosytems Real-Time PCR Tutorial.||Lab Report 3: RT-PCR and cloning|
|Nov. 17||Real-Time PCR Reactions on ABI 7500||Real-Time PCR Protocol|
|Dec. 1||Real-Time PCR Data Analysis||ABI 7500 Tutorial||Lab Report 4: Analysis of YOUR sequence|
|Dec. 8||Course catch-up and wrap-up: discussion of results and check-out.|
|Dec. 19 (Monday)||FINAL REPORT AND NOTEBOOK ARE DUE: 11:30 AM (corresponds to the end of the schedule exam time).||FINAL REPORT DUE 11:30 AM|
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Last Modified Oct. 13, 2005