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Robert A. Oden, Jr. Professor of Biology Department of Biology, Higley Hall Kenyon College 202 North College Road Gambier, Ohio 43022 740-427-5397 slonczewski@kenyon.edu Ultraphyte blog Education Academic Positions Administration and Service Honors Grant Awards Research Publications Science Fiction Interdisciplinary Publications EDUCATION Yale
University, Ph.D.,
Department of Molecular Biophysics and Biochemistry, 1982.
Bryn Mawr College, A.B. in Biology, magna cum laude, 1977. Kenyon
College, Department
of Biology. Robert A. Oden, Jr. Professor,
2014-present; Professor, 2000-2014; Chair of Biology,
1993-1996; Associate Professor, 1991-2000; Assistant
Professor, 1984-1991. U. Maryland at Baltimore, Department of Microbiology and Immunology, Visiting Associate Professor, 1998-1999. Studied pH-regulated virulence factors in Proteus mirabilis and in Helicobacter pylori. Princeton University, Molecular Biology Department, National Science Foundation Visiting Professorship for Women, 1990-1991. Investigated the molecular biology of pH-regulated genes. Created and taught a new course on microbiology. Developed a mentoring program for women scientists. University of Pennsylvania, Department of Biology, Postdoctoral Fellow, National Institutes of Health Research Service Award, 1982-1984. Studied calcium flux in leukocyte chemotaxis. Chair, Faculty Affairs Committee, 2020-2021. Faculty Meeting, Kenyon College. Secretary to the Faculty, Executive Committee of Kenyon College, 2014-2016; 2009-2010; 2001-2002; 1995-1997. Chair of the Faculty, 2006-2007. American Society for Microbiology, Council Policy Committee, At-large District Member, 2007-2010; 2004-2007. Member of Editorial Board, Applied and Environmental Microbiology, 2008-2010. Director, Howard Hughes Medical Institute Undergraduate Biological Sciences Education Program Award, Science Division at Kenyon College, 2004-2008; 2000-2004; 1996-2000. Leading the development of facilities and digital technology in five science departments, three pre-college enrichment programs, and summer science fellowships for minority and first-generation students. Panelist, National Science Foundation, Prokaryotic Genetics Study Section, 2003-2006, 1990-1995. Panelist, NASA Gravitation Ecology and Microbiology, 2002-2003. Chair, Faculty Affairs Committee, 1999-2001. Faculty Meeting, Kenyon College. Chair, Biochemistry and Molecular Biology Program, 1999-2005. Chair of Department of Biology, 1993-1996. Implemented a new major program in Molecular Biology and Biochemistry. Developed course program for non-majors, to complement our research-oriented program for biology majors. Created a comprehensive pre-health advising program. Chair, Facilities Subcommittee of the Campaign Planning Committee, 1996. Developed campaign goal for a new science complex at Kenyon. Director, Elementary School Science Month and Elementary School Scientists program. Implemented hands-on science experiences for grades K-6 at fourteen elementary and middle schools in Mount Vernon City and Knox County, 1992-1994. Chair, Division K. Microbial Physiology and Metabolism, American Society for Microbiology, 1996-1997. Planned program for Annual Meeting. Panelist, Howard Hughes Medical Institute Predoctoral Fellowship Program, Genetics & Molecular Biology, 1995-1997. Faculty
Service Award, Kenyon
College, 2016. Guest of
Honor, International Conference on the Fantastic and the
Arts 36, Orlando,
March 2014. John Campbell Memorial Award for Best Science Fiction Novel, The Highest Frontier, 2012. Division I Lecturer, 109th General Meeting, American Society for Microbiology, Philadelphia, May 2009. Robert Tomsich Award, for outstanding achievement in research in science, Kenyon College, 2001. Vice-Chair, first bi-annual Gordon Conference on Microbial Stress Responses, Plymouth State College, 1994. Wrote and submitted the proposal, with Roberto Kolter. Led sessions or presented invited papers at subsequent meetings, 1996, 1998, 2000, 2002, 2014, 2016. "Mover and Shaker" Award, Mount Vernon News, for the Elementary School Science Program in the Mount Vernon and Knox County elementary schools, 1993. Silver Medalist, National Professor of the Year program, Council for the Advancement and Support of Education, Washington DC, 1989. Vector
Laboratories Young Investigator Travel Award, for outstanding research
presented at the American Society for Microbiology Annual
Meeting, 1987. John Campbell Memorial Award for Best Science Fiction Novel, A Door into Ocean, 1987. National Science Foundation RUI, $540,700. Acid signals and bacterial drug efflux systems, 2019-2022. National Science Foundation, $224,246.
Joan Slonczewski and Wade Powell, Acquisition of FACS cell
sorter for research on antibiotic resistance and environmental
toxicant receptors, 2017-2020. National Science Foundation RUI, $524,000. Benzoate and pH Stress in Experimental Evolution of Escherichia coli, 2016-2019. National Science Foundation RUI, $496,500. Acid and base stress in Escherichia coli, 2013-2016. National Science Foundation RUI, $453,000. Acid and base stress in Escherichia coli, 2011-2013. National Institutes of Health ARRA supplement, $68,118. pH Regulation in Bacillus subtilis, 2009-2010. National Science Foundation RUI, $400,000.
Acid and base stress in Escherichia coli, 2007-2010. Howard Hughes Medical Institute, Undergraduate Biological Sciences Competition, $1,500,000. Student research and teaching, curriculum development and pre-college outreach, 2004-2008. National Science Foundation RUI, $396,000. Acid and base stress in Escherichia coli, 2003-2006. Howard Hughes Medical Institute, Undergraduate Biological Sciences Competition, $700,000. Student research and teaching, curriculum development and pre-college outreach, 2000-2004. National Science Foundation RUI, $370,000. Acid and base stress in Escherichia coli, 2000-2003. Howard Hughes Medical Institute, Undergraduate Biological Sciences Competition, $1,500,000. Student research and teaching, curriculum development and pre-college outreach, 1996-2000. National Science Foundation RUI, $324,000. Acid stress genes in Escherichia coli, 1996-2000. National Science Foundation RUI, $285,000. Acid resistance and stationary phase in Escherichia coli and Shigella flexneri, 1993-1996. Dwight D. Eisenhower Mathematics and Science Education Program, Ohio Board of Regents, $44,200. Elementary Experimental Science Month: Scientists in the Classroom, 1992-1993. Dwight D. Eisenhower Mathematics and Science Education Program, Ohio Board of Regents, $41,621, for Elementary School Scientists, 1993-1994. National Science Foundation, Visiting Professorship for Women, at Princeton University, $77,137, 1990-1991. National Science Foundation RUI, $255,000, pH-regulated genes in E. coli, 1990-1993. National Institutes of Health, direct costs $166,000. "Genes induced by pH perturbation in Escherichia coli," 1987-1990. National Institutes of Health, Academic Research Enhancement Award, $71,869, The genetics of pH Homeostasis in Escherichia coli," 1985-1987. National Science Foundation College Science Instrumentation Program, $25,000, Equipment for Undergraduate Instruction in the Techniques of Subcellular Analysis by Ultracentrifugation, 1985-1987. PUBLICATIONS. *Undergraduate researchers Textbooks:J. L. Slonczewski and John W. Foster. 2023, 2020, 2017, 2014, 2011, 2009. Microbiology: An Evolving Science. Core microbiology textbook for undergraduate science majors. W. W. Norton & Co., New York. John W. Foster, Zarrintaj Aliabadi, and J. L. Slonczewski. 2020, 2018. Microbiology: The Human Experience. Microbiology textbook for nursing and allied health majors. W. W. Norton & Co., New York. Primary Articles: (*Undergraduate coauthors ^High school coauthors) Fitzgerald, Bailey A.,* Ayman Wadud,* Zachary Slimak, and Joan L. Slonczewski. 2023. Enterococcus faecalis OG1RF evolution at low pH selects fusidate-sensitive mutants in elongation factor G and at high pH selects defects in phosphate transport. Appl. Env. Micro. e00466-23. Sheetal Tallada,* Grant Hall,* Daniel Barich, Rachael M. Morgan-Kiss, and Joan L. Slonczewski. 2022. Antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney. Antarctic Science, 34: 408-422. April Murphy,* Daniel Barich, M. Siobhan Fennessy, and Joan L. Slonczewski. 2021. An Ohio State Scenic River shows elevated antibiotic resistance genes, including Acinetobacter tetracycline and macrolide resistance, downstream of wastewater treatment plant effluent. Microbiology Spectrum 9: e00941-21. Samantha H. Schaffner,* Abigail V. Lee,* Minh T. N. Pham,* Beimnet B. Kassaye,* Haofan Li,* Sheetal Tallada,* Cassandra Lis,* Mark Lang,* Yangyang Liu,* Nafeez Ahmed,* Logan G. Galbraith, Katerina M. Bischof, Chelsea C. Menke, and Joan L. Slonczewski. 2021. Extreme acid modulates fitness tradeoffs of multidrug efflux pumps MdtEF-TolC and AcrAB-TolC in Escherichia coli K-12. Appl. Env. Micro. AEM-00724. Zachary D. Blount, Rohan Maddamsetti, Nkruman A Grant, Sumaya T. Ahmed,* Tanush Jagdish, Jessica A. Baxter, Sommerfeld, B.A., Alice Tillman,* Jeremy Moore,* Joan L. Slonczewski, Jeffrey E. Barrick, and Richard E. Lenski. 2020. Genomic and phenotypic evolution of Escherichia coli in a novel citrate-only resource environment. eLife, 9, e55414. DOI: 10.1128/AEM.00966-19. Karina S. Kunka,* Jessie M. Griffith,* Chase Holdener,^ Katarina M. Bischof, Haofan Li,* Priya DasSarma, Shiladitya DasSarma, Joan L. Slonczewski. 2019. Acid and iron experimental evolution of Halobacterium sp. NRC-1. DOI: 10.1101/662882 Jeremy P. Moore,* Haofan Li,* Morgan L. Engmann,* Katarina M. Bischof, Karina S. Kunka,* Mary E. Harris,^ Anna C. Tancredi,* Frederick S. Ditmars,* Preston J. Basting, Nadja S. George, Arvind A. Bhagwat, Joan L. Slonczewski. 2019. Inverted regulation of multidrug efflux pumps, acid resistance and porins in benzoate-evolved Escherichia coli K-12. Appl. Env. Micro., AEM.00966-19; DOI: 10.1128/AEM.00966-19. Jessie M. Griffith,* Preston J. Basting, Katarina M. Bischof, Erintrude P. Wrona,* Karina S. Kunka,* Anna C. Tancredi,* Jeremy P. Moore,* Miriam R. L. Hyman,* and Joan L. Slonczewski. 2019. Experimental evolution of Escherichia coli K-12 in the presence of PMF uncoupler CCCP selects for mutations affecting PMF-driven drug efflux pumps. Appl. Env. Micro. 85:e02792-18; DOI: 10.1128/AEM.02792-18. Mrdjen, Igor, Siobhan Fennessy, Alex Schaal,* Richard Dennis,* Joan L. Slonczewski, Seungjun Lee, and Jiyoung Lee. 2018. Tile drainage and anthropogenic land use contribute to harmful algal blooms and microbiota shifts in inland water bodies. Environmental Science and Technology 52:8215-8223. Hamdallah, Issam,* Nadia Torok,* Katarina M. Bischof, Nadim Majdalani, Sriya Chadalavada,* Nonto Mdluli,* Kaitlin E. Creamer,* Michelle Clark, Chase Holdener,^ Preston J. Basting, Susan Gottesman, and Joan L. Slonczewski. 2018. Experimental evolution of Escherichia coli K-12 at high pH and rpoS induction. Appl. Env. Micro. 84:e00520-18 A. He,* S. R. Penix,* P. J. Basting, J. M. Griffith,* K. E. Creamer,* D. Camperchioli,* M. W. Clark, A. S. Gonzales,* J. S. C. Erazo,* N. S. George, A. A. Bhagwat and J. L. Slonczewski. 2017. Acid evolution deletes amino-acid decarboxylases and reregulates catabolism of Escherichia coli K-12. Appl. Env. Micro. 83: e00442-17. Creamer, K. E.,* F. S Ditmars,* P. J. Basting, K. S. Kunka,* I. N. Hamdallah,* S. P. Bush,* Z. Scott,* A. He,* S. R. Penix,* A. S. Gonzales,* E. K. Eder,* D. Camperchioli,* A. Berndt,* M. W. Clark, K. A. Rouhier, and J. L. Slonczewski. 2017. Benzoate and salicylate tolerant strains lose antibiotic resistance during laboratory evolution of Escherichia coli K-12. Appl. Env. Micro. 83:e02736-16 Clark, M. W., A. M. Yie,* E. K. Eder,* R. G. Dennis,* P. J. Basting, K, A. Martinez II, Brian D. Jones, J. L. Slonczewski. 2015. Periplasmic acid stress increases cell division asymmetry (polar aging) of Escherichia coli. PLoS ONE 10:e0144650. Harden, M. M.,* A. He,* K. Creamer,* M. W. Clark, I. Hamdallah,* K. A. Martinez 2nd, R. L. Kresslein,* S. P. Bush,* J. L. Slonczewski. 2015. Editor Selection. Acid-adapted strains of Escherichia coli K-12 obtained by experimental evolution. Appl. Env. Micro. 81:1932�1941. Riggins, D.P.,* M. J. Narvaez,* K. A. Martinez, M. M. Harden,* and J. L. Slonczewski. 2013. Escherichia coli K-12 survives anaerobic exposure at pH 2 without RpoS, Gad, or hydrogenases, but shows sensitivity to autoclaved broth products. PLoS ONE 8:e56796. Martinez, K. A., R.D. Kitko, J. Patrick Mershon,* H. E. Adcox,*
K. A. Malek,* M. B. Berkmen, and J. L. Slonczewski.
2012. Cytoplasmic pH response to acid stress in individual cells
of Escherichia coli and Bacillus subtilis
observed by fluorescence ratio imaging microscopy. Appl. Env. Micro. 78:3706-3714 Kitko, R. D., J. C. Wilks,* G. M. Garduque,* and J. L. Slonczewski. 2010. Osmolytes enhance pH homeostasis of Escherichia coli. PLoS ONE 5: e10078. Noguchi, K.,* D. P. Riggins,* K. C. Eldahan,* R. D. Kitko, and J. L. Slonczewski. 2010. Hydrogenase-3 contributes to anaerobic acid resistance of Escherichia coli. PLoS ONE 5: e10132. Slonczewski, J. L., J. A. Coker, and S. DasSarma. 2010. Microbial growth with multiple stressors. Microbe 5:110-116. Cover feature. Slonczewski, J. L. M. Fujisawa, M. Dopson, and T. A. Krulwich. 2009. Cytoplasmic pH measurement and homeostasis in bacteria and archaea. Adv. Micro. Physiol. 55:1-79. Request PDF. Kitko, R. D., R. L. Cleeton,* E. I. Armentrout,* G. E. Lee,* K. Noguchi,* M. B. Berkmen, B. D. Jones, and J. L. Slonczewski. 2009. Cytoplasmic Acidification and the benzoate transcriptome in Bacillus subtilis. PLoS ONE 4:e8255. Wilks, J. C.,* R. D. Kitko, S. H. Cleeton,* G. Lee,* C. S.
Ugwu,* B. D. Jones, S. S. BonDurant, and J. L.
Slonczewski. 2009. Acid and base stress and
transcriptomic responses in Bacillus subtilis. Appl. Env. Microbiol.75:
981-990. Wilks, J. C. * and J. L. Slonczewski. 2007. pH of the cytoplasm and periplasm of Escherichia coli: rapid measurement by GFP fluorimetry. J. Bacteriol. 189:5601�5607. Hayes, E. T.,* J.C. Wilks,* E. Yohannes, D. P.
Tate,* M. Radmacher, S. S. BonDurant, and J. L.
Slonczewski. 2006. pH and anaerobiosis coregulate
catabolism, hydrogenases, ion and multidrug transporters, and
envelope composition in Escherichia coli K-12. BMC Microbiology 6:89.
BMC Research Highlight for 2006-2007. Yohannes, E., *A. E. Thurber, *J. C. Wilks, *D. P. Tate, and J. L. Slonczewski. 2005. Polyamine stress at high pH in Escherichia coli K-12. BMC Microbiology 5:59. Maurer, L. M.,* E. Yohannes, S. S. BonDurant, M.
Radmacher, and J. L. Slonczewski. 2005. pH
regulates genes for flagellar motility, catabolism, and
oxidative stress in Escherichia coli K-12. J Bacteriol.
187:304-319. Yohannes, E., D. M. Barnhart,* and J. L. Slonczewski. 2004. pH-Dependent Catabolic Protein Expression during Anaerobic Growth of Escherichia coli K-12. J. Bacteriol. 186:192-199. PDF Stancik, L. M.,* D. M. Stancik,* B. Schmidt, D. M. Barnhart,* Y. N. Yoncheva,* and J. L. Slonczewski. 2002. pH-dependent expression of periplasmic proteins and amino acid catabolism in Escherichia coli. J. Bacteriol. 184:4246-4258. PDF Slonczewski, J. L. and C. Kirkpatrick. 2002. Proteomic analysis of pH-Dependent stress responses in Escherichia coli and Helicobacter pylori, using two-dimensional gel electrophoresis. Methods in Enzymology, Bacterial Pathogenesis Part C, Academic Press. Kirkpatrick, C., L. M. Maurer,* N. E. Oyelakin,* Y. Yontcheva,* R. Maurer, and J. L. Slonczewski. 2001. Acetate and formate stress: opposite responses in the proteome of Escherichia coli. J. Bacteriol. 183:6466-6477. PDF Slonczewski, J. L., D. J. McGee, J. Phillips,* C. Kirkpatrick, and H. L. T. Mobley. 2000. pH-Dependent protein profiles of Helicobacter pylori analyzed by two-dimensional gels. Helicobacter 5:240-247. Blankenhorn, D., Phillips, J.,* Slonczewski, J. L. 1999. Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis. J. Bacteriol. 181: 2209-2216. PDF Lambert, L.,* K. Abshire, D. Blankenhorn, and J. L. Slonczewski. 1997. Global induction of proteins by benzoate at low pH in Escherichia coli. J. Bacteriol. 179:7595-7599. Hersh, B. M.,* F. T. Farooq,* D. N. Barstad,* D. L. Blankenhorn, and J. L. Slonczewski. 1996. A glutamate-dependent acid resistance gene in Escherichia coli. J. Bacteriol. 178:3978-3981. Slonczewski, J. L. and J. W. Foster. 1996. Ch. 96. pH-regulated genes and survival at extreme pH. Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, Frederick C. Neidhardt, Ed. in Chief, American Society for Microbiology Press, Washington D.C., pp. 1539-1549. Lin, J., I. S. Lee, J. Frey,* J. L. Slonczewski, and J. W. Foster. 1995. Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli. J. Bacteriol. 177:4097-4101. Small, P., D. Blankenhorn, D. Welty, E. Zinser,* and J. L. Slonczewski. 1994. Acid and base resistance in Escherichia coli and Shigella flexneri: the role of rpoS and growth pH. J. Bacteriol. 176: 1729-1737. Rosner, J. L., and Slonczewski, J. L. 1994. Dual regulation of inaA by the multiple antibiotic resistance (Mar) and superoxide (SoxRS) stress response systems of Escherichia coli. J. Bacteriol. 176:6262-6269 Lee, I. S., J. L. Slonczewski, and J. W. Foster. 1994. A low pH-inducible stationary phase acid tolerance response in S. typhimurium. J. Bacteriol. 176:1422-1426. White, S., F. E. Tuttle,* D. Blankenhorn, D. C. Dosch, and J. L. Slonczewski. 1992. pH dependence and gene structure of inaA in Escherichia coli. J. Bacteriol. 174:1537-1543. Watson, N., D. S. Dunyak, E. L. Rosey, J. L. Slonczewski, and E. R. Olson. 1992. Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH. J. Bacteriol. 174:530-540. Bingham, R. J.,* K. S. Hall, and J. L. Slonczewski. 1990. Alkaline induction of a novel gene locus alx-1 in Escherichia coli. J. Bacteriol. 172:2184-2186. Aliabadi, Z., Y. K. Park, J. L. Slonczewski, and J. W. Foster. 1988. Novel regulatory loci controlling oxygen- and pH-regulated gene expression in Salmonella typhimurium. J. Bacteriol. 170:842-851. Zigmond, S. H., J. L. Slonczewski, M. W. Wilde, and M. Carson. 1988. Polymorphonuclear leukocyte locomotion is insensitive to lowered cytoplasmic calcium levels. Cell Motility and the Cytoskeleton 9:184-189. Slonczewski, J. L., T. N. Gonzalez,* F. M. Bartholomew, and N. J. Holt. 1987. Mud-directed lacZ fusions regulated by acid pH in Escherichia coli. J. Bacteriol. 169:3001-3006. Slonczewski, J. L., M. W. Wilde, and S. H. Zigmond. 1985. Phosphorylase a activity as an indicator of neutrophil activation by chemotactic peptides. J. Cell Biol. 101:1191-1197. Slonczewski, J. L., R. M. Macnab, J. R. Alger, and A. Castle. 1982. Effects of pH and repellent tactic stimuli on protein methylation levels in Escherichia coli. J. Bacteriol. 152:384-399. Slonczewski, J. L., B. P. Rosen, J. R. Alger, and R. M. Macnab. 1981. pH homeostasis in Escherichia coli: Measurement by P31 nuclear magnetic resonance of methylphosphonate and phosphate. Proc. Natl. Acad. Sci. USA 78:6271-6275. The Highest Frontier. College out in space�Invaded by undocumented aliens. Tor/Macmillan, September, 2011. Brain Plague. Intelligent microbes invade human brains, offering limitless powers--at a price. Tor Books, August, 2000; Science Fiction Book of the Month Club, Alternate Selection. The Children Star. A planet with biochemistry so alien that only children can be genetically engineered to survive there. And what unique alien intelligence is watching in secret? Analog serial, April, 1998; Tor Books, September, 1998. Daughter of Elysium. In the far future, biologists engineer humans to live for thousands of years, then face a revolt by the machines that made it possible. Avon, 1993; Easton Press signed first edition, 1993; Avon pbk, 1994. The Wall around Eden. Quaker teen-agers face the environmental consequences of nuclear war. William Morrow, 1989; Avon pbk, 1990; Italian translation, Editrice Nord, 1991. A Door into Ocean. Women biologists genetically engineer fantastic creatures on a planet covered entirely by ocean. Science Fiction Book of the Month Club Main Selection, 1986; Arbor House, 1986; Avon pbk, 1987; Italian translation, Editrice Nord, 1988. Still Forms on Foxfield. Quakers colonize a planet with bizarre alien inhabitants. DelRey Books, 1980. INTERDISCIPLINARY ARTICLES AND CHAPTERS J. L. Slonczewski and R. Marusak. 2004. A response to BIO 2010: Transforming Undergraduate Education for Future Research Biologists, from the perspective of the Biochemistry and Molecular Biology Major Program at Kenyon College. Biochem. Mol. Biol. Ed. 32:151-155. J. L. Slonczewski and M. Levy. 2003. �Chapter 12: Science Fiction and the Life Sciences,� in The Cambridge Companion to Science Fiction, edited by E. James and F. Mendlesohn, Cambridge University Press, Cambridge, UK. �Joan Slonczewski: Stranger than fiction.,� 2003. Profile in Nature 426:501. J. L. Slonczewski. 2002. �Stem cells and human cloning,� Analog Science Fiction and Science Fact, November, pp. 26-29. J. L. Slonczewski. 2000. �Tuberculosis bacteria join UN,� Nature 405:1001. J. L. Slonczewski, S. Lindow, M. Levy, C. Jacobsen, and K. Hellekson. 2000. �Approaching The Children Star,� conference report with questions for writing and discussion, Science Fiction Research Association Review, July/August pp. 17-27. J. L. Slonczewski. 1994. �Science in science fiction: making it work,� The Writer, pp 14-17. J. L. Slonczewski. 1986. �A tale of two handmaids,� review of A Handmaid�s Tale by Margaret Atwood and The Magnificent Spinster by May Sarton, The Kenyon Review, New Series 8:120-124. |
