- Finding Genes. The National
Center for Biotechnology (NCBI)
provides many tools for identifying sequence.
- Genbank
Input words such as "tryptophanase" and "Escherichia" to find sequence
of interest.
- BLAST
Use BLAST if you know some sequence, and want to find other similar
sequences, either in E. coli or other organisms.
- Kenn
Rudd's Ecogene Find your gene on the map, along
with its sequence, and possible regulators.
- Ecocyc
for metabolic pathways
- Analyzing DNA
- Primer design for PCR.
- Primer3 Primer
design and analysis program. Once you know which gene regulatory
region to amplify, use this site to design primers.
- Operon Order DNA
oligos here.
- Literature search.
- Medline
Search literature for reports of your gene, including lac fusion
and knock-out mutants.
- ASM Journals
Search J. Bacteriology and other ASM journals. Any article
found here we can download (see Dr. S. or Chris).
- Proteomics
|
