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When pK19 is cut by EcoRI it has "sticky ends" that are complementary to those made by cutting R. sphaeroides. Like R. sphaeroides the "sticky ends" can reanneal if DNA ligase is added. This would return the plasmid to it's original ring structure.

Step 2. Mix cut pK19 with cut R. sphaeroides DNA.

When pK19 is cut by EcoRI it has "sticky ends" that are complementary to those made by cutting R. sphaeroides. Like R. sphaeroides the "sticky ends" can reanneal if DNA ligase is added. This would return the plasmid to it's original ring structure.

Step 2. Mix cut pK19 with cut R. sphaeroides DNA.