R. sphaeroides
DNA containing the gene of interest
R. sphaeroides
cut ends of DNA
pK19 DNA
Note the complementarity of the "sticky ends" of the pK19 and the
R. sphaeroides
DNA.
Step2.
Cool, add DNA ligase and the molecules can reanneal. Resulting in a variety of recombinant forms.
The one of interest is the
plasmid containing the
R. sphaeroides
DNA.