Helicobacter  pylori  26695

pH-Dependent Proteins

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Gels performed by: Judith Phillips and Chris Kirkpatrick
Web links by BIOL 56 Experimental Genetics students at Kenyon College:
Catherine Beck, Neala Kendall, and Dan Nickerson
Contributors from U. Maryland at Baltimore:
David McGee, and Harry L. T. Mobley
Site director: Joan L. Slonczewski
Grant support: NSF MCB 9630963
References
Last Revision Date, December 29, 1999

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Click on each image for spot map.

Acid growth	Alkaline growth
pH 6.1 ESA pI range = 4.5-6.5 Coomassie Blue stain	pH 7.5 ESA pI range = 4.5-6.5 Coomassie Blue stain
pH 5.9 IPGphor pI range = 4.5-9.0 Silver stain	pH 7.5 IPGphor pI range = 4.5-9.0 Silver stain

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Growth conditions: Yeast extract-tryptone broth, 3% fetal bovine serum,
buffered with 100 mM sulfonate buffer appropriate for pH
First dimension ESA tube gels were run using procedure by ESA (Genomic Solutions).
First dimension IPGphor strips were 3-10 Nonlinear, by AP Biotech.
All second-dimension gels were 11.5 % acrylamide SDS-PAGE, using ESA Duracryl.

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