Fall Semester 2006
Activities subject to the uncertainties of science.
Deadlines are firm.
Updated Aug 9, 2006
NOTE: Readings and handouts are available by direct link or via e-res, the electronic reserve system of the CONSORT libraries.
|Aug. 30||Course introduction; pipettor verification; quantification of nucleic acids by spectrophotometry; intro. to degenerate primer design||Morrison et al. (1995)
Morrison et al. (1998)
Iwata and Stegeman (2000)
Wilkie and Simon (1991)
|Sept. 6||Total RNA Isolation, quantitation.||RNA isolation protocol|
|Sept. 13||RNA gels, photography; Figure preparation;
Workshop: Primer design.
|Wilkie and Simon (1991)||Homework: primer sequences|
(Meet in Computer Lab, FSH 009)
|Computer Workshop: Bioinformatics BLAST searches, sequence alignment, phylogenetic trees.||
Handout: DNA and Protein Sequence analysis.
|Lab Report 1: RNA Isolation
Also: Notebook check.
|Sept. 27||RT-PCR for CYP1A cDNA fragments||RT-PCR Protocols|
|Oct. 4||Agarose gel analysis of PCR products|
|Oct. 11||Purification of RT-PCR products from gels; ligation of products into plasmid cloning vector.||Gene Clean protocol
|Lab Report 2: Sequence analysis exercise.|
|Oct. 18 (and 19)||Transformation of competent E. coli cells.|
|Oct. (24 and) 25||Plasmid minipreps; restriction enzyme digestion.||Miniprep protocol
Restriction ezyme protocol
|Notebook Check by request.|
|Nov. 1||Gel analysis of miniprep digests.
Workshop: Intro to Real-Time PCR;
|Real-Time PCR Data Analysis Tutorial.||Lab Report 3: RT-PCR and cloning|
|Nov. 15||Real-Time PCR Reactions on ABI 7500||Real-Time PCR Protocol|
|Nov. 29||Real-Time PCR Data Analysis||ABI 7500 Tutorial||Lab Report 4: Analysis of YOUR sequence|
|Dec. 6||Course catch-up and wrap-up: discussion of results and check-out.|
|Dec. 16 (Saturday)||FINAL REPORT AND NOTEBOOK ARE DUE: 4:30 PM (corresponds to the end of the schedule exam time).||FINAL REPORT DUE 4:30 PM|
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Last Modified Aug. 9, 2006