KAP Genes and Development
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Last modified 03/23/2007 MWF 10:10-11:00
PM (Period 4) Dr. Wade Powell FSH 202, x5396 powellw[a]kenyon.edu Office hours: MW, 11-1; T, 4-5; R, 10-11 and by appointment This course introduces the mechanisms of heredity, the expression of genetic information, and the genetic control of development. Special emphasis is placed on genetic and biochemical approaches toward delineating fundamental cellular and developmental processes.
How to do well in this course.
Grades. Grades are based on:
Academic Honesty. Do not lie, cheat, or plagiarize. This class will follow the official Kenyon College position on academic honesty. Please review and observe the official College policy on academic honesty. Specific instructions on take-home quizzes or other unique assignments will be provided. Special Needs. College policy provides for reasonable accomodations for documented physical, psychological or learning disabilities that may impact your ability to participate fully in the course. Please speak with me and with Erin Salva, Coordinator of Disability Services (PBX 5453; salvae@kenyon.edu). All information and documentation of disability is confidential. Please see the Disability Services web site for more information. Logistical details of any approved accommodation (e.g. extended exam time) must be arranged with the instructor at least 3 days in advance. |
DATE | TOPIC & WEBNOTES links | READING -- Color coded |
Part I: Heritability
of Genotype and Phenotype
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Jan 15
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Course
introduction; history
of genetics; genes and genomes.
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Jan 17 |
Ch. 18 pp. 383-386; Ch. 3, p 130 (nucleoid) and p. 133 (nucleus) |
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Jan 19 | Cell Division QUIZ 1 | Ch. 11 pp. 227-238; Ch. 12 pp. 248-267; Web tutorials 11.1 and 12.1 |
Jan 22 | Mendelian inheritance | Ch. 13 pp. 269-281; Web tutorials 13.1 and 13.2; Dihybrid Cross; Download Flowers Software |
Jan 24 |
Ch. 13 pp. 281-282, 294-297; Sex-linked inheritance; X and Y |
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Jan 26 |
Recombination
and gene mapping; |
Ch. 13 pp 283-287; Linkage |
Jan
29
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Ch. 13 pp. 287-293 |
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Jan 31 | Genetics Catch up |
More practice problems |
Feb 2 | DNA as the genetic material | Ch. 14 pp. 303-307 |
Feb 5 | EXAM 1 | |
Part II: Genes and
Gene Expression
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Feb 7 | DNA
structure; practice DNA bases |
Ch. 4 pp. 74-83; Web tutorial 4.1 Classic papers |
Feb 9
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DNA
replication; Quiz 3
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Ch. 14 pp. 307-318; Ch. 7, p. 140 (Box 7.1); Web tutorial 14.1
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Feb 12 |
Ch. 4 pp. 83-85; Ch. 15 pp. 325-328, 334-336; Ch. 16
pp. 338-341; Web tutorials 15.1 and 16.1;
Beadle and Tatum animation
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Feb 14
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Ch. 15 pp. 328-333; Web
tutorial 15.2
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Feb 16 | Ch. 3 pp. 48-56; Ch. 16 pp. 345-355; Web tutorial 16.2 | |
Feb 19 | Eukaryotic
transcription and splicing PLOS Biology: choosing splice sites |
Ch. 16 pp. 342-345 Splice mechanism: Robinson (2006) PLOS Biology |
Feb
21
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Ch. 12 pp. 264-266; Ch. 14 pp. 318-323; Ch. 16 pp.
355-359; Web tutorial 12.2
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Feb 23 |
Applying Central Dogma Concepts I: Regulation of the eukaryotic cell cycle. QUIZ 5 |
Ch. 11 pp. 227-232, 238-245; Web tutorial 11.1; Cell cycle game |
Feb
26
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Ch. 18 pp. 395-398; Smoking
and Lung Cancer
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Feb 28 | Applying Central Dogma Concepts II: Immune System and antibody production | Ch. 49 pp. 1124-1132 |
Mar 2 | EXAM 2 | ------- |
Mar 3-18
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SPRING BREAK
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No Class Meetings
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Part III: Regulation
of gene expression
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Mar 19 |
Problems
and Approaches I:
1. PCR: Making lots of DNA from just a little; 2. How to determine and annotate DNA sequence |
Ch. 19 pp. 408-411, Ch. 20 pp. 426-439, Web tutorials 19.2 and 20.1; PCR
animation
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Mar 21 |
Problems and
Approaches II: Customizing DNA molecules. Recombinant
DNA, transgenic
organisms, and gene therapy. |
Ch. 4 pg. 78; Ch. 19 pp. 401-407, 411-424; Web tutorial 19.1; Restriction enzyme animation; Plasmid cloning animation; Plasmid (downloaded with Flowers); puzzler |
Mar 23 |
Problems and Approaches III: Measuring Gene Expression: northern blots, RT-PCR, western blots, QUIZ 6 |
Ch. 19 pp. 412-413; |
Mar 26
(Dr. S) |
Ch. 20 pp. 439-443; DNA
Chip; New Yorker Article.
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Mar 28 (Dr. S) |
Ch. 17 pp. 362-379 |
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Mar 30 |
Prokaryotic Gene Expression II: Operon Analysis; Regulons, Promoters QUIZ 7 |
Ch. 17 pp. 362-379; Web tutorial 17.1 |
Apr
2
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Ch. 18 pp. 382-390
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Apr 4 | Eukaryotic Gene Expression II: Classes and functions of transcription factors; Analysis of regulatory motifs | Ch. 18 pp. 386-391; Web tutorial 18.1 |
Apr 6 |
Eukaryotic Gene
Expression III: |
Ch. 18 pp. 384-386; 392-395 |
Apr 9 | Junk
DNA? non-coding RNAs and gene expression RNAi movie |
RNAi and riboswitches: Gibbs (2003) Scientific American article (local access only) |
Apr 11
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EXAM 3
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Part IV: Developmental
Biology
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Apr 13 | Overview of Developmental Processes; Gene Expression and Development | Ch. 21 pp. 461-467; Ch. 22 pp. 481-484 |
Apr 16 | Microbial development and more microbial development; | Heterocysts |
Apr 18 | Fertilization and Cleavage in animals |
Ch. 21 pp. 446-458 |
Apr 20 |
Cell
Movement and Gastrulation; QUIZ 9
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Ch. 8, 159-168; Ch. 21 pp. 458-461; Web tutorials 21.1 and 21.2 |
Apr 23
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Ch. 21 pp. 466-467; Ch 22
pp. 484 and 489
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Apr 25 | Pattern formation in Animals | Ch. 21 pp. 461, 463-464; Ch. 22 pp. 469-478; Web tutorial 22.1; Animated tutorial |
Apr 27 | Gene Duplication and Molecular Evolution: Hox Genes QUIZ 10 | Ch. 22 pp. 476-478; Ch. 26 pp. 569-572. C. elegans, Twinned genes |
April
30
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Cell Differentiation: Neurulation and organogenesis. | Ch. 22 pp. 484-489 |
May 2 |
Ch. 21 pp. 452-453, 462-463;
Ch. 22 pp. 478-481
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May 4 | Catch-up; Course Evaluation | ------------ |
May 7 | EXAM 4 | 8:30 AM! |